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PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations

In this study, we describe the development of a PCR-free whole exome sequencing method. Using this method, 2 μg DNA was sufficient for library preparation for whole exome sequencing. Furthermore, the method is simple and makes use of a commercial kit, with additional step of concentrating the captur...

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Detalhes bibliográficos
Publicado no:PLoS One
Principais autores: Yamaguchi, Izumi, Watanabe, Takashi, Ohara, Osamu, Hasegawa, Yoshinori
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6743761/
https://ncbi.nlm.nih.gov/pubmed/31518370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0222562
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