A Genetically Encoded Ratiometric pH-Probe: Wavelength Regulation-Inspired Design of pH Indicators

Mutants of human Cellular Retinol Binding Protein II (hCRBPII) were engineered to bind a julolidine retinal analog for the purpose of developing a ratiometric pH sensor. The design relied on the electrostatic influence of a titratable amino acid side chain, affecting the absorption, and thus emissio...

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Publicat a:Chembiochem
Autors principals: Berbasova, Tetyana, Nick, Setare Tahmasebi, Nosrati, Meisam, Nossoni, Zahra, Santos, Elizabeth M., Vasileiou, Chrysoula, Geiger, James H., Borhan, Babak
Format: Artigo
Idioma:Inglês
Publicat: 2018
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6742514/
https://ncbi.nlm.nih.gov/pubmed/29645331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201800050
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