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Quantitative detection of ALK fusion breakpoints in plasma cell-free DNA from patients with non-small cell lung cancer using PCR-based target sequencing with a tiling primer set and two-step mapping/alignment

BACKGROUND: Tyrosine kinase inhibitors targeted to anaplastic lymphoma kinase (ALK) have been demonstrated to be effective for lung cancer patients with an ALK fusion gene. Application of liquid biopsy, i.e., detection and quantitation of the fusion product in plasma cell-free DNA (cfDNA), could imp...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Kunimasa, Kei, Kato, Kikuya, Imamura, Fumio, Kukita, Yoji
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6742348/
https://ncbi.nlm.nih.gov/pubmed/31513617
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0222233
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