Effect of diluent type, cryoprotectant concentration, storage method and freeze/thaw rates on the post-thaw quality and fertility of cryopreserved alpaca spermatozoa

This study compared protocols for cryopreservation of ejaculated, papain-treated alpaca spermatozoa. This included different concentrations of egg yolk (EY; 5, 10 or 15%) and glycerol (2, 5 or 10%), diluent types (SHOTOR, lactose, skim milk or INRA-96™), freeze rates (2, 4 or 8 cm above liquid nitro...

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Publicat a:Sci Rep
Autors principals: Stuart, C. C., Vaughan, J. L., Kershaw, C. M., de Graaf, S. P., Bathgate, R.
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2019
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6731240/
https://ncbi.nlm.nih.gov/pubmed/31492923
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-49203-z
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