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Genetic Engineering by DNA Recombineering
Recombineering inserts PCR products into DNA using homologous recombination. A pair of short homology arms (50 base pairs) on the ends of a PCR cassette target the cassette to its intended location. These homology arms can be easily introduced as 5′ primer overhangs during the PCR reaction. The flex...
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| Vydáno v: | Curr Protoc Chem Biol |
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| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2019
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6727988/ https://ncbi.nlm.nih.gov/pubmed/31483098 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpch.70 |
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