Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

In this article, we present a protocol that is optimized to preserve neutrophil-lineage cells in fresh BM for whole BM CyTOF analysis. We utilized a myeloid-biased 39-antibody CyTOF panel to evaluate the hematopoietic system with a focus on the neutrophil-lineage cells by using this protocol. The Cy...

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Veröffentlicht in:J Vis Exp
Hauptverfasser: Zhu, Yanfang Peipei, Padgett, Lindsey, Dinh, Huy Q., Marcovecchio, Paola, Wu, Runpei, Hinz, Denise, Kim, Cheryl, Hedrick, Catherine C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6726111/
https://ncbi.nlm.nih.gov/pubmed/31282876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59617
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