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Optimisation of robust singleplex and multiplex droplet digital PCR assays for high confidence mutation detection in circulating tumour DNA

Liquid biopsies offer the potential to monitor cancer response and resistance to therapeutics in near real-time. However, the plasma cell free DNA (cfDNA) level can be low and the fraction of circulating tumour DNA (ctDNA) bearing a mutation – lower still. Detection of tumour-derived mutations in ct...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Rowlands, Vicky, Rutkowski, Andrzej J., Meuser, Elena, Carr, T. Hedley, Harrington, Elizabeth A., Barrett, J. Carl
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6718424/
https://ncbi.nlm.nih.gov/pubmed/31477768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-49043-x
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