A Novel Mismatched PCR-Restriction Fragment Length Polymorphism Assay for Rapid Detection of gyrA and parC Mutations Associated With Fluoroquinolone Resistance in Acinetobacter baumannii

BACKGROUND: Mutations in the quinolone resistance-determining regions (QRDRs) of Acinetobacter baumannii DNA gyrase (gyrA) and topoisomerase IV (parC) are linked to fluoroquinolone (FQ) resistance. We developed a mismatched PCR-restriction fragment length polymorphism (RFLP) assay to detect mutation...

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Bibliografiske detaljer
Udgivet i:Ann Lab Med
Main Authors: Kakuta, Naoki, Nakano, Ryuichi, Nakano, Akiyo, Suzuki, Yuki, Tanouchi, Ayako, Masui, Takashi, Horiuchi, Saori, Endo, Shiro, Kakuta, Risako, Ono, Yasuo, Yano, Hisakazu
Format: Artigo
Sprog:Inglês
Udgivet: The Korean Society for Laboratory Medicine 2020
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Original Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6713654/
https://ncbi.nlm.nih.gov/pubmed/31432636
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3343/alm.2020.40.1.27
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