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β-mercaptoethanol assists efficient construction of sperm bacterial artificial chromosome library

Bacterial artificial chromosome (BAC) library plays a critical role in the strategic research in genomics. Sperm is known as a good source for BAC library construction. However, preparation of intact DNA from the highly condensed sperm nuclei is not easy. Here we developed and validated an efficient...

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Detalhes bibliográficos
Publicado no:J Biol Methods
Main Authors: Fujikura, Kohei, Abe, Masanori, Kuroda, Reiko
Formato: Artigo
Idioma:Inglês
Publicado em: jbm 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6708924/
https://ncbi.nlm.nih.gov/pubmed/31453223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2017.167
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