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Analysis of lipoprotein transport depletion in Vibrio cholerae using CRISPRi

Genes necessary for the survival or reproduction of a cell are an attractive class of antibiotic targets. Studying essential genes by classical genetics, however, is inherently problematic because it is impossible to knock them out. Here, we screened a set of predicted essential genes for growth inh...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Caro, Florence, Place, Nicole M., Mekalanos, John J.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2019
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6708369/
https://ncbi.nlm.nih.gov/pubmed/31371515
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1906158116
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