CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

BACKGROUND: Pichia pastoris (syn. Komagataella phaffii) is a widely used generally recognized as safe host for heterologous expression of proteins in both industry and academia. Recently, it has been shown to be a potentially good chassis host for the production of high-value pharmaceuticals and che...

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Bibliographic Details
Published in:Microb Cell Fact
Main Authors: Liu, Qi, Shi, Xiaona, Song, Lili, Liu, Haifeng, Zhou, Xiangshan, Wang, Qiyao, Zhang, Yuanxing, Cai, Menghao
Format: Artigo
Language:Inglês
Published: BioMed Central 2019
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Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6704636/
https://ncbi.nlm.nih.gov/pubmed/31434578
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-019-1194-x
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