A strand-specific real-time quantitative RT-PCR assay for distinguishing the genomic and antigenomic RNAs of Rift Valley fever Phlebovirus

Rift Valley Fever Phlebovirus (RVFV), genus Phlebovirus, family Phenuiviridae, order Bunyavirales, has a single-stranded, negative-sense RNA genome, consisting of L, M and S segments. Here, we report the establishment of a strand-specific, quantitative reverse transcription (RT)-PCR assay system tha...

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Udgivet i:J Virol Methods
Main Authors: Tercero, Breanna, Terasaki, Kaori, Nakagawa, Keisuke, Narayanan, Krishna, Makino, Shinji
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6698219/
https://ncbi.nlm.nih.gov/pubmed/31315022
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2019.113701
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