Analysis of Paramyxovirus Transcription and Replication by High-Throughput Sequencing

We have developed a high-throughput sequencing (HTS) workflow for investigating paramyxovirus transcription and replication. We show that sequencing of oligo(dT)-selected polyadenylated mRNAs, without considering the orientation of the RNAs from which they had been generated, cannot accurately be us...

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Detalhes bibliográficos
Publicado no:J Virol
Main Authors: Wignall-Fleming, Elizabeth B., Hughes, David J., Vattipally, Sreenu, Modha, Sejal, Goodbourn, Steve, Davison, Andrew J., Randall, Richard E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2019
Assuntos:
Genome Replication and Regulation of Viral Gene Expression
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6694822/
https://ncbi.nlm.nih.gov/pubmed/31189700
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.00571-19
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