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Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus

CRISPR-Cas systems are now widely used for genome editing and transcriptional regulation in diverse organisms. The compact and portable nature of class 2 single effector nucleases, such as Cas9 or Cas12, has facilitated directed genome modifications in plants, animals, and microbes. However, most CR...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Hidalgo-Cantabrana, Claudio, Goh, Yong Jun, Pan, Meichen, Sanozky-Dawes, Rosemary, Barrangou, Rodolphe
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6690032/
https://ncbi.nlm.nih.gov/pubmed/31341082
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1905421116
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