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Investigating the Deoxyribonuclease Activity of CRM197 with Site-Directed Mutagenesis

[Image: see text] The protein cross-reactive material 197 (CRM197) is known to catalyze the hydrolytic cleavage of DNA (DNase activity). A suspected metal-binding site (S109, T111, and E112) and suspected DNA-binding motif (T89, K90, and V91) were predicted within the CRM197 protein X-ray crystal st...

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Detalhes bibliográficos
Publicado no:ACS Omega
Main Authors: Bravo-Bautista, Nathalie, Hoang, Hieu, Joshi, Anusha, Travis, Jennifer, Wooten, Melissa, Wymer, Nathan J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2019
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6682014/
https://ncbi.nlm.nih.gov/pubmed/31460310
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.9b00418
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