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Highly efficient construction of infectious viroid-derived clones
BACKGROUND: Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low efficiency, (ii) it is a non-oriented reacti...
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| Veröffentlicht in: | Plant Methods |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6670230/ https://ncbi.nlm.nih.gov/pubmed/31388344 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-019-0470-4 |
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