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Reconstitution of Clathrin Coat Disassembly for Fluorescence Microscopy and Single-Molecule Analysis.

The disassembly of the clathrin lattice surrounding coated vesicles is the obligatory last step in their life cycle. It is mediated by the coordinated recruitment of auxilin and Hsc70, an ATP-driven molecular clamp. Here, we describe the preparation of reagents and the single-particle fluorescence m...

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Publicado en:Methods Mol Biol
Main Authors: Böcking, Till, Upadhyayula, Srigokul, Rapoport, Iris, Capraro, Benjamin R, Kirchhausen, Tom
Formato: Artigo
Idioma:Inglês
Publicado: 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6669891/
https://ncbi.nlm.nih.gov/pubmed/30129014
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8719-1_10
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