High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics

Since G-protein coupled receptors (GPCRs) are linked to various diseases, screening of functional ligands against GPCRs is vital for drug discovery. In the present study, we developed a high-throughput functional cell-based assay by combining human culture cells producing a GPCR, yeast cells secreti...

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Publicado en:Sci Rep
Main Authors: Yaginuma, Kenshi, Aoki, Wataru, Miura, Natsuko, Ohtani, Yuta, Aburaya, Shunsuke, Kogawa, Masato, Nishikawa, Yohei, Hosokawa, Masahito, Takeyama, Haruko, Ueda, Mitsuyoshi
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2019
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6662714/
https://ncbi.nlm.nih.gov/pubmed/31358824
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-47388-x
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