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Induction of T7 Promoter at Higher Temperatures May Be Counterproductive
Bacterial expression of recombinant proteins is the most popular and convenient method for obtaining large quantities of pure protein. The induction of T7 promoter with isopropyl-β-d-thiogalactopyranoside (IPTG) is widely used for expression of large quantities of proteins in Escherichia coli. It ha...
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| 發表在: | Indian J Clin Biochem |
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| Main Authors: | , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Springer India
2019
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6660524/ https://ncbi.nlm.nih.gov/pubmed/31391729 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12291-019-0813-y |
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