One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus

To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 10(2.1) TCID(50) for CDV, 10(1.9) TCID(50) for CPV and 10(3) copies for CaKoV. This method did not amp...

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Bibliografiske detaljer
Udgivet i:J Vet Med Sci
Main Authors: LIU, Dafei, LIU, Fei, GUO, Dongchun, HU, Xiaoliang, LI, Zhijie, LI, Zhigang, MA, Jianzhang, LIU, Chunguo
Format: Artigo
Sprog:Inglês
Udgivet: The Japanese Society of Veterinary Science 2018
Fag:
Virology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6656820/
https://ncbi.nlm.nih.gov/pubmed/29367517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1292/jvms.17-0442
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