High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes

High-throughput single-cell RNA sequencing is a powerful technique but only generates short reads from one end of a cDNA template, limiting the reconstruction of highly diverse sequences such as antigen receptors. To overcome this limitation, we combined targeted capture and long-read sequencing of...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Singh, Mandeep, Al-Eryani, Ghamdan, Carswell, Shaun, Ferguson, James M., Blackburn, James, Barton, Kirston, Roden, Daniel, Luciani, Fabio, Giang Phan, Tri, Junankar, Simon, Jackson, Katherine, Goodnow, Christopher C., Smith, Martin A., Swarbrick, Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6635368/
https://ncbi.nlm.nih.gov/pubmed/31311926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-11049-4
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