A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos

BACKGROUND: The insulated isothermal PCR (iiPCR) technology enables consistent PCR amplification and detection in a simple heating device. A pan-dengue virus (DENV) RT-iiPCR, targeting the 5’ untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Tsai, Jih-Jin, Liu, Wei-Liang, Lin, Ping-Chang, Huang, Bo-Yi, Tsai, Ching-Yi, Lee, Pei-Yu Alison, Tsai, Yun-Long, Chou, Pin-Hsing, Chung, Simon, Liu, Li-Teh, Chen, Chun-Hong
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2019
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6619671/
https://ncbi.nlm.nih.gov/pubmed/31291289
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0218139
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