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Non-transgenic marker-free gene disruption by an episomal CRISPR system in the oleaginous microalga, Nannochloropsis oceanica CCMP1779

Utilization of microalgae has been hampered by limited tools for creating loss-of-function mutants. Furthermore, modified strains for deployment into the field must be free of antibiotic resistance genes and face fewer regulatory hurdles if they are transgene free. The oleaginous microalga, Nannochl...

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Vydáno v:Plant J
Hlavní autoři: Poliner, Eric, Takeuchi, Tomomi, Du, Zhi-Yan, Benning, Christoph, Farré, Eva M.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6616531/
https://ncbi.nlm.nih.gov/pubmed/29518315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/tpj.14314
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