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B‐factor Guided Proline Substitutions in Chromobacterium violaceum Amine Transaminase: Evaluation of the Proline Rule as a Method for Enzyme Stabilization

Biocatalysis is attracting interest in the chemical industry as a sustainable alternative in large‐scale chemical transformations. However, low operational stability of naturally evolved enzymes is a challenge and major efforts are required to engineer protein stability, usually by directed evolutio...

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Detalhes bibliográficos
Publicado no:Chembiochem
Principais autores: Land, Henrik, Campillo‐Brocal, Jonatan C., Svedendahl Humble, Maria, Berglund, Per
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6593452/
https://ncbi.nlm.nih.gov/pubmed/30637901
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201800749
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