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A simplified workflow for monoclonal antibody sequencing
The diversity of antibody variable regions makes cDNA sequencing challenging, and conventional monoclonal antibody cDNA amplification requires the use of degenerate primers. Here, we describe a simplified workflow for amplification of IgG antibody variable regions from hybridoma RNA by a specialized...
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| Pubblicato in: | PLoS One |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Public Library of Science
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6590890/ https://ncbi.nlm.nih.gov/pubmed/31233538 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0218717 |
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