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Isolation of thermally sensitive protein-binding oligonucleotides on a microchip

This paper presents a microfluidic chip capable of isolating thermally sensitive protein-binding aptamer candidates. The chip makes use of bead-immobilized target molecules and DNA (deoxyribonucleic acid) sequences to enable a simplified chip design, in which affinity selection and PCR (polymerase c...

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Détails bibliographiques
Publié dans:Microfluid Nanofluidics
Auteurs principaux: Hilton, John P., Olsen, Timothy, Kim, Jinho, Zhu, Jing, Nguyen, ThaiHuu, Barbu, Mihaela, Pei, Renjun, Stojanovic, Milan, Lin, Qiao
Format: Artigo
Langue:Inglês
Publié: 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6586242/
https://ncbi.nlm.nih.gov/pubmed/31223297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10404-015-1604-2
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