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A Counterselectable Sucrose Sensitivity Marker Permits Efficient and Flexible Mutagenesis in Streptococcus agalactiae

Streptococcus agalactiae (group B Streptococcus [GBS]) is a cause of severe infections, particularly during the newborn period. While methods exist for generating chromosomal mutations in GBS, they are cumbersome and inefficient and present significant challenges if the goal is to study subtle mutat...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Hooven, Thomas A., Bonakdar, Maryam, Chamby, Anna B., Ratner, Adam J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6585494/
https://ncbi.nlm.nih.gov/pubmed/30658970
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03009-18
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