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Primer specificity in the polyadenylation of poly- and oligonucleotides by purified poly(A) polymerase

Poly(A) polymerase (E.C. 2.7.7.19) was purified from bovine liver nuclei by the use of chromatography on a heparin-immobilized Sepharose 4B. The specificity of this enzyme to primers was studied with poly- and oligo-nucleotides. The presence of KCl limited the enzyme action on the long poly(A) or Es...

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Pubblicato in:Nucleic Acids Res
Autori principali: Fukami, Hiroichi, Ohyama, Yuji, Ohta, Takahisa
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 1978
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6581317/
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/1.suppl_2.s435
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