Novel kidney dissociation protocol and image-based flow cytometry facilitate improved analysis of injured proximal tubules

Flow cytometry studies on injured kidney tubules are complicated by the low yield of nucleated single cells. Furthermore, cell-specific responses such as cell cycle dynamics in vivo have conventionally relied on indirect immunohistochemistry and proximal tubule markers that may be downregulated in i...

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Detalhes bibliográficos
Publicado no:Am J Physiol Renal Physiol
Main Authors: Manolopoulou, Marika, Matlock, Brittany K., Nlandu-Khodo, Stellor, Simmons, Alan J., Lau, Ken S., Phillips-Mignemi, Melanie, Ivanova, Alla, Alford, Catherine E., Flaherty, David K., Gewin, Leslie S.
Formato: Artigo
Idioma:Inglês
Publicado em: American Physiological Society 2019
Assuntos:
Innovative Methodology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6580245/
https://ncbi.nlm.nih.gov/pubmed/30759021
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajprenal.00354.2018
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