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Aplysia synaptosomes. I. Preparation and biochemical and morphological characterization of subcellular membrane fractions

We prepared and characterized subcellular membrane fractions from the CNS of Aplysia californica that are enriched in isolated nerve terminals (synaptosomes). Ganglia were homogenized in 1.1 M sucrose and fractionated on a 2-step sucrose gradient, yielding 50 micrograms protein/animal in the synapto...

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Bibliografische gegevens
Gepubliceerd in:J Neurosci
Hoofdauteurs: Chin, GJ, Shapiro, E, Vogel, SS, Schwartz, JH
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Society for Neuroscience 1989
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6570005/
https://ncbi.nlm.nih.gov/pubmed/2913212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1523/JNEUROSCI.09-01-00038.1989
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