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Aplysia synaptosomes. I. Preparation and biochemical and morphological characterization of subcellular membrane fractions
We prepared and characterized subcellular membrane fractions from the CNS of Aplysia californica that are enriched in isolated nerve terminals (synaptosomes). Ganglia were homogenized in 1.1 M sucrose and fractionated on a 2-step sucrose gradient, yielding 50 micrograms protein/animal in the synapto...
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| Publicado no: | J Neurosci |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Society for Neuroscience
1989
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6570005/ https://ncbi.nlm.nih.gov/pubmed/2913212 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1523/JNEUROSCI.09-01-00038.1989 |
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