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Solubilization of the apamin receptor associated with a calcium- activated potassium channel from rat brain
The apamin binding protein was solubilized from rat brain synaptic membranes using sodium cholate. Receptor yield and stability depended closely on the detergent/protein ratio. In optimum conditions the receptor retained high affinity for mono 125I-iodoapamin with Kd = 40 pM at pH 7.5 and 1 degree C...
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| Publicado no: | J Neurosci |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Society for Neuroscience
1987
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6568909/ https://ncbi.nlm.nih.gov/pubmed/2434630 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1523/JNEUROSCI.07-02-00565.1987 |
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