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rEXPAR: An Isothermal Amplification Scheme That Is Robust to Autocatalytic Parasites

[Image: see text] In the absence of DNA, a solution containing the four deoxynucleotidetriphosphates (dNTPs), a DNA polymerase, and a nicking enzyme generates a self-replicating mixture of DNA species called parasite. Parasites are problematic in template-based isothermal amplification schemes such...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Urtel, Georg, Van Der Hofstadt, Marc, Galas, Jean-Christophe, Estevez-Torres, André
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2019
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6562758/
https://ncbi.nlm.nih.gov/pubmed/31074259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.9b00063
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