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Use of nested PCR for the detection of trichomonads in bronchoalveolar lavage fluid

BACKGROUND: The methods routinely used to detect trichomonads in the lungs are not sensitive enough, and an effective method is urgently needed. METHOD: Primers were first designed to match the conserved area of the 18S rRNA gene of trichomonads. Then, nested PCR was carried out to detect trichomona...

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Publicado en:BMC Infect Dis
Autores principales: Lin, Chao, Ying, Furong, Lai, Yanan, Li, Xiaolong, Xue, Xiangyang, Zhou, Tieli, Hu, Dongwei
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6558733/
https://ncbi.nlm.nih.gov/pubmed/31182037
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12879-019-4118-9
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