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Improved method for isolating high-quality RNA from mouse bone with RNAlater at room temperature

Accurate gene expression analysis of bone requires the ability to isolate RNA of good quality. Isolation of intact RNA from frozen bone tissue is problematic since RNA rapidly becomes degraded after thawing. Since we are interested in assessing gene expression from both bone marrow and mineralized b...

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Detalhes bibliográficos
Publicado no:Bone Rep
Main Authors: Pedersen, Kim B., Williams, Ashlee, Watt, James, Ronis, Martin J.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6558217/
https://ncbi.nlm.nih.gov/pubmed/31198821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bonr.2019.100211
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