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Microfluidic System for In-Flow Reversible Photoswitching of Near-Infrared Fluorescent Proteins

We have developed a microfluidic flow cytometry system to screen reversibly photoswitchable fluorescent proteins for contrast and stability of reversible photoconversion between high- and low-fluorescent states. A two-color array of 20 excitation and deactivation beams generated with diffractive opt...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Anal Chem
Hauptverfasser: Lychagov, Vladislav V., Shemetov, Anton A., Jimenez, Ralph, Verkhusha, Vladislav V.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6554737/
https://ncbi.nlm.nih.gov/pubmed/27807973
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.6b03499
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