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NATF (Native and Tissue-Specific Fluorescence): A Strategy for Bright, Tissue-Specific GFP Labeling of Native Proteins in Caenorhabditis elegans

GFP labeling by genome editing can reveal the authentic location of a native protein, but is frequently hampered by weak GFP signals and broad expression across a range of tissues that may obscure cell-specific localization. To overcome these problems, we engineered a Native And Tissue-specific Fluo...

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Publicat a:Genetics
Autors principals: He, Siwei, Cuentas-Condori, Andrea, Miller, David M.
Format: Artigo
Idioma:Inglês
Publicat: Genetics Society of America 2019
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6553825/
https://ncbi.nlm.nih.gov/pubmed/30952669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.119.302063
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