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Systematic evaluation of the microRNAome through miR-CATCHv2.0 identifies positive and negative regulators of BRAF-X1 mRNA

Here we present miR-CATCHv2.0, an implemented experimental method that allows the identification of the microRNA species directly bound to an RNA of interest. After cross-linking of microRNA::RNA::Ago2 complexes using formaldehyde, the RNA is fragmented using sonication and then subjected to affinit...

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Bibliografische gegevens
Gepubliceerd in:RNA Biol
Hoofdauteurs: Marranci, Andrea, D’Aurizio, Romina, Vencken, Sebastian, Mero, Serena, Guzzolino, Elena, Rizzo, Milena, Pitto, Letizia, Pellegrini, Marco, Chiorino, Giovanna, Greene, Catherine M., Poliseno, Laura
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Taylor & Francis 2019
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6546394/
https://ncbi.nlm.nih.gov/pubmed/30929607
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2019.1600934
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