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Systematic evaluation and optimization of the experimental steps in RNA G-quadruplex structure sequencing

cDNA library preparation is important for many high-throughput sequencing applications, such as RNA G-quadruplex structure sequencing (rG4-seq). A systematic evaluation of the procedures of the experimental pipeline, however, is lacking. Herein, we perform a comprehensive assessment of the 5 key exp...

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Dettagli Bibliografici
Pubblicato in:Sci Rep
Autori principali: Yeung, Pui Yan, Zhao, Jieyu, Chow, Eugene Yui-Ching, Mou, Xi, Hong, HuiQi, Chen, Leilei, Chan, Ting-Fung, Kwok, Chun Kit
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2019
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6542847/
https://ncbi.nlm.nih.gov/pubmed/31147619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-44541-4
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