High-throughput targeted gene deletion in the model mushroom Schizophyllum commune using pre-assembled Cas9 ribonucleoproteins

Efficient gene deletion methods are essential for the high-throughput study of gene function. Compared to most ascomycete model systems, gene deletion is more laborious in mushroom-forming basidiomycetes due to the relatively low incidence of homologous recombination (HR) and relatively high inciden...

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Vydáno v:Sci Rep
Hlavní autoři: Jan Vonk, Peter, Escobar, Natalia, Wösten, Han A. B., Lugones, Luis G., Ohm, Robin A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2019
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6529522/
https://ncbi.nlm.nih.gov/pubmed/31113995
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-44133-2
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