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Kinetics of Transient Protein Complexes Determined via Diffusion-Independent Microfluidic Mixing and Fluorescence Stoichiometry
Low-affinity protein complexes and their transient states are difficult to measure in single-molecule experiments because of the requirement of low concentrations. A prominent solution to this problem is the use of microfluidic mixing devices, which rely on diffusion-based mixing of fluorescently la...
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| Publicado no: | J Phys Chem B |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6526119/ https://ncbi.nlm.nih.gov/pubmed/30351113 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jpcb.8b07437 |
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