Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry

Membrane proteins reside in lipid bilayers and are typically extracted from this environment for study, which often compromises their integrity. Here we eject intact assemblies from membranes, without chemical disruption, and use mass spectrometry to define their composition. From E. coli outer memb...

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Detalhes bibliográficos
Publicado no:Science
Main Authors: Chorev, Dror S., Baker, Lindsay A., Wu, Di, Beilsten-Edmands, Victoria, Rouse, Sarah L., Zeev-Ben-Mordehai, Tzviya, Jiko, Chimari, Samsudin, Firdaus, Gerle, Christoph, Khalid, Syma, Stewart, Alastair G., Matthews, Stephen J., Grünewald, Kay, Robinson, Carol V.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6522346/
https://ncbi.nlm.nih.gov/pubmed/30442809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.aau0976
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