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Patch clamp and perfusion techniques to study ion channels expressed in Xenopus oocytes

The Xenopus oocyte expression system is ideal for electrophysiological characterization of voltage-dependent and ligand-dependent ion channels due to its relatively low background of endogenous channels and the large size of the cell. Here we present a protocol to study voltage and ligand dependent...

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Detalhes bibliográficos
Publicado no:Cold Spring Harb Protoc
Main Authors: Zhang, Guohui, Cui, Jianmin
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6522140/
https://ncbi.nlm.nih.gov/pubmed/29382809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot099051
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