Calcium phosphate mineralization in bone tissues directly observed in aqueous liquid by atmospheric SEM (ASEM) without staining: microfluidics crystallization chamber and immuno-EM

The malformation and disordered remodeling of bones induce various diseases, including osteoporosis. We have developed atmospheric SEM (ASEM) to directly observe aldehyde-fixed bone tissue immersed in radical scavenger buffer without thin sectioning. The short procedure realized the observation of b...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Sato, Chikara, Yamazaki, Daiju, Sato, Mari, Takeshima, Hiroshi, Memtily, Nassirhadjy, Hatano, Yuri, Tsukuba, Takayuki, Sakai, Eiko
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6517404/
https://ncbi.nlm.nih.gov/pubmed/31089159
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-43608-6
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