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TROSY Pulse Sequence for Simultaneous Measurement of the (15)N R(1) and {(1)H} -(15)N NOE in Deuterated Proteins

A TROSY-based NMR experiment is described for simultaneous measurement of the (15)N longitudinal relaxation rate constant R(1) and the {(1)H}-(15)N nuclear Overhauser enhancement. The experiment is based on the observation that the TROSY mixing pulse sequence element symmetrically exchanges (1)H and...

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Vydáno v:J Biomol NMR
Hlavní autoři: O’Brien, Paul A., Palmer, Arthur G.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6510663/
https://ncbi.nlm.nih.gov/pubmed/29663108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-018-0181-6
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