Single Shot Capillary Zone Electrophoresis-Mass Spectrometry Produces Over 27,000 Peptide and nearly 4,400 Protein Identifications by Combining a Very Low-Electroosmosis Coated Capillary, a Third-Generation Electrokinetically-Pumped Sheath-Flow Nanospray Interface, an Orbitrap Fusion Lumos Tribid Mass Spectrometer, and an Advanced Peak Determination Algorithm

We show that capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) generates very large numbers of peptide and protein identifications (IDs) by combining four technologies: a separation capillary coated to generate very low electroosmosis, an electrokinetica...

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Detalhes bibliográficos
Publicado no:Anal Chem
Main Authors: Zhang, Zhenbin, Hebert, Alexander S., Westphall, Michael S., Qu, Yanyan, Coon, Joshua J., Dovichi, Norman J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6508074/
https://ncbi.nlm.nih.gov/pubmed/30179504
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.8b02991
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