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Single Shot Capillary Zone Electrophoresis-Mass Spectrometry Produces Over 27,000 Peptide and nearly 4,400 Protein Identifications by Combining a Very Low-Electroosmosis Coated Capillary, a Third-Generation Electrokinetically-Pumped Sheath-Flow Nanospray Interface, an Orbitrap Fusion Lumos Tribid Mass Spectrometer, and an Advanced Peak Determination Algorithm
We show that capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) generates very large numbers of peptide and protein identifications (IDs) by combining four technologies: a separation capillary coated to generate very low electroosmosis, an electrokinetica...
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| Publicado no: | Anal Chem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6508074/ https://ncbi.nlm.nih.gov/pubmed/30179504 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.8b02991 |
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