Capillary zone electrophoresis-tandem mass spectrometry for large-scale phosphoproteomics with the production of over 11000 phosphopeptides from the colon carcinoma HCT116 cell line

Phosphoproteomics requires better separation of phosphopeptides to boost the coverage of the phosphoproteome. We argue that an alternative separation method that produces orthogonal phosphopeptide separation to the widely used LC needs to be considered. Capillary zone electrophoresis (CZE) is one im...

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Udgivet i:Anal Chem
Main Authors: Chen, Daoyang, Ludwig, Katelyn R., Krokhin, Oleg V., Spicer, Vic, Yang, Zhichang, Shen, Xiaojing, Hummon, Amanda B., Sun, Liangliang
Format: Artigo
Sprog:Inglês
Udgivet: 2019
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6506858/
https://ncbi.nlm.nih.gov/pubmed/30624053
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.8b04770
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