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Evaluation of a duplex reverse-transcription real-time PCR assay for the detection of encephalomyocarditis virus

We evaluated a fluorogenic probe–based assay for the detection of encephalomyocarditis virus (EMCV) by comparing a set of published primers and probe to a new set of primers and probe. The published reagents failed to amplify a range of Australian isolates and an Italian reference strain of EMCV. In...

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Библиографические подробности
Опубликовано в: :J Vet Diagn Invest
Главные авторы: Qin, Shaomin, Underwood, Darren, Driver, Luke, Kistler, Carol, Diallo, Ibrahim, Kirkland, Peter D.
Формат: Artigo
Язык:Inglês
Опубликовано: SAGE Publications 2018
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC6505904/
https://ncbi.nlm.nih.gov/pubmed/29860932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1040638718779112
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