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Long DCL4-substrate dsRNAs efficiently induce RNA interference in plant cells

RNA interference (RNAi) is induced by the direct transfer of double-stranded RNAs (dsRNAs) into protoplasts prepared from Arabidopsis thaliana seedlings. In this protoplast RNAi system, we compared the efficacies of various-sized dsRNAs (between 21 and 139 nucleotides [nt]) for inducing RNAi and ass...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Kakiyama, Sayaka, Tabara, Midori, Nishibori, Yuki, Moriyama, Hiromitsu, Fukuhara, Toshiyuki
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6502814/
https://ncbi.nlm.nih.gov/pubmed/31061468
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-43443-9
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