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Deleterious effects of formalin-fixation and delays to fixation on RNA and miRNA-Seq profiles

The National Cancer Institute conducted the Biospecimen Pre-analytical Variables (BPV) study to determine the effects of formalin fixation and delay to fixation (DTF) on the analysis of nucleic acids. By performing whole transcriptome sequencing and small RNA profiling on matched snap-frozen and FFP...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Jones, Wendell, Greytak, Sarah, Odeh, Hana, Guan, Ping, Powers, Jason, Bavarva, Jasmin, Moore, Helen M.
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6502812/
https://ncbi.nlm.nih.gov/pubmed/31061401
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-43282-8
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