Epitope tagging of chromosomal genes in Salmonella

We have developed a simple and efficient procedure for adding an epitope-encoding tail to one or more genes of interest in the bacterial chromosome. The procedure is a modification of the gene replacement method of Datsenko and Wanner [Datsenko, K. A. & Wanner, B. L. (2000) Proc. Natl. Acad. Sci...

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Bibliografiske detaljer
Main Authors: Uzzau, Sergio, Figueroa-Bossi, Nara, Rubino, Salvatore, Bossi, Lionello
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences 2001
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC65018/
https://ncbi.nlm.nih.gov/pubmed/11742086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.261348198
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